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我们试用标记分析法,用3种单克隆抗体(黄病毒科特异性、登革病毒属特异性和登革病毒2型型特异性单克隆抗体)研究了我国海南省1985～1987年3年内流行的登革2型病毒8个流行株的抗原性变化,并与标准株新几内亚B株进行了比较。发现6株与标准株类似,2株显示出明显的差异。标记分析法为病毒抗原分析提供了一个简便、快速的方法,可用来监测一个地区病毒株群的变化及新株的传入。
Abstract：Defining the extent of antigenic variation between dengue-2 viruses from Hainan province was tried, which had been isolated over a period of three years(1985-1987). The dengue-2 viruses were compared with the prototype New Guinea B strain and were subjected to antigen signature analysis. Eight strains of dengue-2 virus were analyzed by three monoclonal antibodies, flaviviridae, dengue-virus and dengue-2 type specific reactive epitopes, over a range of antigen concentration. Six out of eight of dengue-2 virus were shown to be antigenically homogeneous, and the remaining two strains, to be heterogeneous. Signature analysis provides a rapid and simple means to differentiate strains derived from different sources, thus permiting monitoring changes or introduction of new dengue virus populations in certain geographic region.
1 Monath TP, et al. Geographic classification of dengueu-2 virus strains by antigen signature analysis.Virology 1986;154 (2) : 313
2 辛颜彬,等.登革2型病毒单克隆抗体的制备与鉴定.军事医学科学院院刊1986;10(1) :59
3 Raker PJ, et al. Protein and cell mmebrane iodinations with a sparingly soluble chloroamide,1,3,4,6-tetrachloro-3a,6a-diphenylglycoluril.Biochem Biophys Commun 1978;80(4) : 849
4 杨佩英,等.辛得比斯病毒结构多肽的抗原性.军事医学科学院院刊 1983;7(5) :509
5 杨佩英,等.用标记分析法对我国海南地区登革2型病毒株的抗原表位分析.微生物学报 1989;29(4) :299
基本信息:
引用信息:
[1]司炳银,杨佩英,徐品芳,李瑞霞,陈文洲,阎国珍.用标记分析法检测我国海南省登革2型病毒抗原性变化[J].细胞与分子免疫学杂志,1990(01):27-30.
1990-04-02
1990-04-02