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目的 探讨苍耳子(XF)活性成份通过自噬-代谢重编程促进过敏性鼻炎(AR)大鼠鼻黏膜屏障修复的实验研究。方法 将24只雌性Sprague-Dawley大鼠(SD;5周龄)随机分为对照组、 AR组和XF组，每组8只。建立卵清蛋白(OVA)诱导的AR大鼠模型。观察大鼠的行为变化，Western blot法检测NOD样受体家族含pyrin结构域3(NLRP3)、线粒体外膜前蛋白转位酶20(TOM20)、微管相关蛋白1轻链3B(LC3B)、选择性自噬接头蛋白1(P62)、 PTEN诱导假定激酶1(PINK1)、帕金蛋白(Parkin)、白细胞介素1β(IL-1β)、 IL-18蛋白表达。人鼻上皮细胞系(HNEpC)分组处理如下：空白组(Con)、 IL-13组、 IL-13联合XF组、 IL-13和XF联合si-PINK1组。使用流式细胞术评估细胞死亡。结果 与对照组相比，AR组大鼠打喷嚏的次数和鼻部抓痕的数量显著增加。与AR组相比，XF组大鼠打喷嚏的次数和鼻部抓痕的数量显著减少。AR组大鼠出现鼻黏膜纤毛的损伤和炎性细胞浸润，XF减少了这些病理变化。与对照组相比，AR组大鼠鼻黏膜组织中NLRP3、 IL-1β、 IL-18、 PINK1、 Parkin、 LC3BⅡ/LC3BⅠ表达水平显著增加，P62蛋白表达显著减少。与AR组相比，XF组大鼠鼻黏膜组织中PINK1、 Parkin、 LC3BⅡ/LC3BⅠ蛋白表达进一步增加，NLRP3、 IL-1β、 IL-18、 P62蛋白表达水平显著降低。与Con组相比，IL-13组HNEpC中NLRP3、 IL-1β、 IL-18、 PINK1、 Parkin、 LC3BⅡ/LC3BⅠ水平显著升高，TOM20蛋白表达显著减少。与IL-13组相比，IL-13联合XF组HNEpC中NLRP3、 IL-1β、 IL-18、 P62、 TOM20蛋白表达水平显著降低，PINK1、 Parkin、 LC3BⅡ/LC3BⅠ蛋白表达进一步增加。si-PINK1敲低PINK1蛋白逆转了XF对IL-13暴露的HNEpC的保护作用。结论 XF可能通过激活PINK1-Parkin介导的线粒体自噬作用抑制NLRP3炎性小体的激活，有助于减轻细胞死亡并促进鼻黏膜屏障修复，从而缓解AR症状。
Abstract：Objective To investigate the effect of active components of Xanthii Fructus(XF) on the repair of nasal mucosal barrier in allergic rhinitis(AR) rats through autophagy-metabolic reprogramming. Methods 24 female Sprague-Dawley rats(SD; 5 weeks old) were randomly divided into control group, AR group and XF group, with 8 rats in each group. an AR rat model induced by ovalbumin(OVA) was established. The behavioral changes of rats were observed, and Western blot analysis revealed the expression levels of the NOD-like receptor family contained pyrin domain 3(NLRP3), mitochondrial outer membrane protein transposase 20(TOM20), microtubule-associated protein 1 light chain 3B(LC3B), selective autophagy linker 1(P62), PTEN-induced putative kinase 1(PINK1), Parkin(Parkin), interleukin 1β(IL-1β) and IL-18. Human nasal epithelial cell lines(HNEpCs) were divided into the following groups: blank group(Con), IL-13 group, IL-13+XF group and IL-13+XF+si-PINK1 group. Cell death was evaluated by flow cytometry. Results Compared with the control group, thetimes of sneezing and the number of nasal scratches in AR group increased significantly. Compared with AR group, the sneezing times and the number of nasal scratches in XF group reduced significantly. In AR group, nasal cilia injury and inflammatory cell infiltration occurred in rats, and XF reduced these pathological changes. Compared with the control group, the expression levels of NLRP3, IL-1β, IL-18, PINK1, Parkin and LC3BⅡ/LC3BⅠ in the nasal mucosa of rats in AR group increased significantly, while the expression of P62 protein decreased significantly. Compared with AR group, the expressions of PINK1, Parkin, LC3BⅡ/LC3BⅠ proteins in the nasal mucosa of XF group were further increased, while the expressions of NLRP3, IL-1β, IL-18 and P62 proteins were significantly decreased. Compared with Con group, the levels of NLRP3, IL-1β, IL-18, PINK1, Parkin and LC3B Ⅱ/LC3B Ⅰ in HNEpCs in IL-13 group increased significantly, and the expression of TOM20 protein decreased significantly. Compared with IL-13 group, the expression levels of NLRP3, IL-1β, IL-18, P62 and TOM20 in HNEpCs in IL-13+XF group were significantly decreased, while the expression levels of PINK1, Parkin and LC3B Ⅱ/LC3B Ⅰ were further increased. si-PINK1 reversed the protective effect of XF on HNEpCs exposed by IL-13. Conclusion XF may inhibit the activation of NLRP3 inflammatory corpuscles by activating mitochondrial autophagy mediated by PINK1-Parkin, which is helpful to reduce apoptosis and promote the repair of nasal mucosal barrier, thus alleviating AR symptoms.
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基本信息:
DOI：10.13423/j.cnki.cjcmi.010053
中图分类号:R285.5
引用信息:
[1]仇顺锋,成虎.苍耳子活性成份通过自噬-代谢重编程促进变应性鼻炎大鼠鼻黏膜屏障修复的实验研究[J].细胞与分子免疫学杂志,2026,42(01):28-35.DOI:10.13423/j.cnki.cjcmi.010053.
基金信息:
江苏省中医药科技发展计划项目(MS2022139)
2025-09-17
2025-09-17
2025-09-17